Table 2. Primary antibodies used and pretreatment
Marker Clone Antibody type Source Dilution Pretreatment

CA125 M11 M Fujirebio Diagnostics, Malvern, PA Prediluted No

EMA E29 M Dako Corporation, Carpinteria, CA 1:800 No

Cytokeratin AE1/AE3 M Dako Corporation, Carpinteria, CA 1:300 Trypsynization

CEA CEM010 M Mochida Pharmaceutical, Tokyo 1:400 Trypsynization

Vimentin V9 M Dako Corporation, Carpinteria, CA 1:1000 Autoclave

CD34 NU-4A1 M Nichirei Corporation, Tokyo 1:50 No

Antibody type M: monoclonal antibody.