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Japanese Journal of Clinical Oncology 16:253-260 (1986)
© 1986 Foundation for Promotion of Cancer Research

research-article

Chemosensitivity Test for Human Small Cell Lung Cancer Cell Lines In Vitro

HIDENOBU TAKAHASHI, M.D.1,3,, NAGAHIRO SAIJO, M.D.2, TETSU SHINKAI, M.D.2, KENJI EGUCHI, M.D.2, YASUTSUNA SASAKI, M.D.2, TOMOHIDE TAMURA, M.D.2, TETSURO SANO, M.D.2, HIDEHIKO NAKANO, M.D.1, KAZUHIKO NAKAGAWA, M.D.1, MASANORI SAKURAI, M.D.2, WEON-SEON HONG, M.D.4, AKIO HOSHI, Ph.D.1 and YOSHIHIRO HAYATA, M.D.3

1Pharmacology Division Tokyo
2Department of internal Medicine, National Cancer Center Research institute and Hospital Tokyo
3Department of Surgery, Tokyo Medical College Tokyo
4Korea Cancer Center Hospital Seoul

Reprint requests: Hidenobu Takahashi, M.D., Pharmacology Division, National Cancer Center Research Institute, 1-1, Tsukiji chome, Chuo-ku, Tokyo 104, Japan.

Received August 22, 1986; The in vitro response to seven chemotherapeutic drugs of three established human small cell lung cancer (SCLC) cell lines (NCI H69, H 128, N231) was tested by a double soft agar clonogenic assay. Colony formation by the three cell lines was universally reduced more than 50% by continuous exposure to peak plasma concentrations of all the drugs. However by exposure to one-tenth of the peak plasma concentrations, the colony growth of H69 was reduced to 25.6% and 37.7% by etoposide and teniposide, respectively, and that of N231 was reduced to 46.7%, 39.0%, 27.5% by carboplatin, etoposide and tenipo side, respectively. On the other hand colony formation by the three cell lines was not suppressed more than 50% by one-hour exposure to any of the drugs tested at one-tenth of the peak plasma concentrations. By one-hour exposure to drugs at the peak plasma concentrations, colony formation by H69, H128 and N23 I was reduced more than 50% by cisplatin, etoposide, teniposide and nimustin, by adriamycin, tenoposide and ACNU, and by adriamycin, etoposide, teniposide and nimustin, respectively. It was concluded that these three cell lines have similar sensitivity to seven drugs commonly used against small cell lung cancer.

Key Words: Small cell lung cancer • Chemosensitivity • Clonogenic assay


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