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Japanese Journal of Clinical Oncology 21:8-12 (1991)
© 1991 Foundation for Promotion of Cancer Research

research-article

Human Papillomavirus Type 31 DNA Detected in Part of the Dysplasia but in No Part of the Squamous Metaplasia in a Specimen Taken from One Patient

Satoshi Shimano1,*, Michio Fukushima1,2, Akira Nishikawa1, Yasushi Yamakawa1, Satoru Takashima1, Masaaki Satoh3, Takashi Minase4, Takeshi Kutuzwa5 and Masayoshi Hashimoto1

1Department of Obstetrics and Gynecology, Sapporo Medical College Minami 1-jo, Nishi 16-chome, Chuo-ku, Sappro 060
2Department of Molecular Biology, Cancer Research Institute, Sapporo Medical College Minami 1-jo, Nishi 16-chome, Chuo-ku, Sapporo 060
3Division of Pathology, Sapporo Medical College Hospital Minami 1-jo, 291 Nishi 16-chome, Chuo-ku, Sap poro 060
4Department of Pathology, Sapporo NTT Hospital Minami 1-jo, 290 Nishi 15-chome, Chuo-ku, Sapporo 060
5Hokkaido Cancer Society Kita-2-jo, Nishi 12-jo, Chuo-Ku, Sapporo 060

*For reprints and all correspondence

Received June 4, 1990; accepted October 2, 1990

Using in situ hybridization, human papillomavirus (HPV 6, 16, 18, 31, 33) DNAs were detected in a cervical severe dysplasia accompanied by squamous metaplasia. It was found that, only HPV 31 DNA was harbored in the cervical severe dysplasia, but HPV DNAs were not identified in a lesion of squamous metaplasia. The in situ hybridization method will be of use, therefore, when dysplasia with squamous metaplasia or other lesions are examined for HPV DNA. In a cervical smear, HPV 31 DNA could be detected on the nuclei of dysplastic cells, so this method is applicable to cervical smears. If squamous metaplasia is to be considered as a precursor lesion to cervical dysplasia, the HPV DNA harbored in the dysplasia must also be detected in the accompanying squamous metaplasia. Our results suggested that not all squamous metaplasias were involved with HPV, as far as we were able to detect using five types of HPV DNA probe.

Key Words: Cervical dysplasia • HPV 31 DNA • Squamous metaplasia • In situ hybridization


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