Japanese Journal of Clinical Oncology, Vol 27, Issue 1 1-5, Copyright © 1997 by Foundation for Promotion of Cancer Research
S Mizobuchi, H Sakamoto, Y Tachimori, H Kato, H Watanabe and M Terada
To elucidate the association of human papillomavirus (HPV) and Epstein-Barr
virus (EBV) with carcinogenesis of the esophagus, 41 surgically resected
specimens and 12 cell lines of esophageal squamous cell carcinoma were
examined for the presence of HPV DNA and EBV DNA by polymerase chain
reaction using primers for the E6 regions of HPV-16 and -18 and for the
EBNA 1 region of EBV. We designed the reaction condition to amplify HPV and
EBV DNA specifically and detected by gel electrophoresis. In ethidium
bromide staining, no band was detected either for the HPV E6 region of for
the EBV EBNA 1 gene in any of the surgically resected specimens and the
cell lines, although the HPV sequence was detectable by Southern blot
hybridization, which is a more sensitive detection method than staining;
three out of 41 surgically resected specimens were positive for HPV-18 by
Southern blot hybridization of polymerase chain reaction products. However,
the number of viral genomes has been estimated as lower than 1 x 10(-3)
copies per cell based on the intensity of the hybridization signals.
Moreover, the DNA samples extracted from the corresponding non-cancerous
esophageal mucosa were also positive for HPV-18, and the intensities of the
hybridization signals were almost the same as those of the tumors. The
results of our study indicate that HPV-16, HPV-18 and EBV are not generally
associated with esophageal carcinogenesis.
ORIGINAL ARTICLE
Absence of human papillomavirus-16 and -18 DNA and Epstein-Barr virus DNA in esophageal squamous cell carcinoma
Genetics Division, National Cancer Center Research Institute, Tokyo, Japan.
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