Japanese Journal of Clinical Oncology, Vol 27, Issue 1 13-17, Copyright © 1997 by Foundation for Promotion of Cancer Research
T Nishijima, M Nishina and K Fujiwara
Proton nuclear magnetic resonance (1H-NMR) has been utilized for
qualitative and quantitative measurement of the components of
nonhomogeneous biological specimens, as it can analyze sensitively the
chemical structure of organic compounds without pretreatment of the
materials. Levels of lactate in serum and bile were measured by 1H-NMR in
healthy volunteers and patients with non-malignant or malignant diseases of
the liver and biliary tract, and the usefulness of such measurements for
the diagnosis of hepatobiliary malignancies was determined. The mean (+/-
SD) serum lactate levels were 0.52 +/- 0.33 mmol/l in five healthy
volunteers, 1.38 +/- 1.59 mmol/l in 30 patients with non-malignant diseases
and 2.95 +/- 2.00 mmol/l in 21 patients with malignant diseases, the
differences among the three groups being significant. Biochemical enzymatic
measurement of serum lactate levels revealed no such difference. In bile,
the spectrum of lactate was observed in all of 16 patients with malignant
diseases, but in none of two healthy volunteers and 12 patients with
non-malignant diseases. The mean time required for the measurement was
36.77 min for serum and 6.40 min for bile. The measurement of lactate
levels in serum and bile using 1H-NMR may be useful for the detection of
hepatobiliary malignancies.
ORIGINAL ARTICLE
Measurement of lactate levels in serum and bile using proton nuclear magnetic resonance in patients with hepatobiliary diseases: its utility in detection of malignancies
Third Department of Internal Medicine, Saitama Medical School, Japan.
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