Japanese Journal of Clinical Oncology, Vol 29, Issue 2 58-62, Copyright © 1999 by Foundation for Promotion of Cancer Research
H Yamamoto, F Itoh, Y Adachi, H Fukushima, H Itoh, S Sasaki, Y Hinoda and K Imai
BACKGROUND: The prognosis of patients with hepatocellular carcinoma is
relatively poor because of the high rate of intrahepatic recurrences. We
have previously demonstrated an association between enhanced secretion of
active matrix metalloproteinases (MMPs; gelatinase A and matrilysin) and
early recurrence in hepatocellular carcinoma. The aim of this study was to
examine further the relationship between messenger RNA levels of
metalloproteinases and their inhibitors and behavior of this carcinoma.
METHODS: Messenger RNA expression of gelatinase A, gelatinase B, matrilysin
and tissue inhibitor of metalloproteinases (TIMP)-1 and TIMP-2 were
analyzed in 30 patients with hepatocellular carcinoma by semiquantitative
reverse transcription-polymerase chain reaction (RT-PCR). The results were
contrasted with the clinicopathological data of the patients. RESULTS:
Enhanced mRNA expression of gelatinase A, gelatinase B and matrilysin in
tumor was observed in 20, 22 and 19 of 30 patients, respectively. Enhanced
mRNA expression of gelatinase A or gelatinase B and of matrilysin showed
trends toward presence of capsular invasion (P = 0.078) and intrahepatic
metastasis (P = 0.064), respectively. Concomitant overexpression of
gelatinase A and matrilysin was associated with portal invasion,
intrahepatic metastasis and recurrence within the first postoperative year
(P < 0.05). A modest increase of mRNA expression of TIMP-1 and TIMP-2 in
tumor was observed in half of the patients, but did not correlate with any
clinicopathological features. CONCLUSION: Our results suggest that
semiquantitative RT-PCR analysis of MMPs may be helpful in disease
management of patients with hepatocellular carcinoma.
ORIGINAL ARTICLE
Messenger RNA expression of matrix metalloproteinases and tissue inhibitors of metalloproteinases in human hepatocellular carcinoma
Department of Internal Medicine, Sapporo Medical University, Japan.
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