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Japanese Journal of Clinical Oncology 30:472-477 (2000)
© 2000 Foundation for Promotion of Cancer Research

Clinical Significance of Major and Minor bcr/abl Chimeric Transcripts in Essential Thrombocythemia

Hironori Yamada1, Takaya Murakami2, Tsuguhiro Kaneda2, Mayumi Tadachi2, Makoto Utsumi1, Saburo Minami3, Motohiro Hamaguchi3, Masanobu Kasai3, Yoshihisa Kodera3, Haruhiko Ohashi4, Yoshihisa Morishita5, Teruhiko Terasawa1, Yumiko Yamasaki1, Yoshikazu Kamiya1, Mamiko Hattori1, Katsuo Yamanaka1, Keitaro Tsushita1 and Masanori Shimoyama1,+,§

1Department of Hematology and 2Department of Clinical Research, Nagoya National Hospital, Nagoya, 3Department of Hematology, Nagoya First Red Cross Hospital, Nagoya, 4First Department of Internal Medicine, Nagoya University, Nagoya and 5Department of Hematology, Kohnan Showa Hospital, Kohnan, Aichi, Japan

Background: Contradictory results have been reported in terms of detecting bcr/abl transcripts in patients with essential thrombocythemia. The aim of this study was to investigate whether the bcr/abl transcript could be found in patients with essential thrombocythemia (ET).

Methods: The bcr/abl transcript was amplified by the RT-nested PCR method using RNA extract from leukocytes taken from 14 essential thrombocythemia patients. The amplified DNAs were electrophoresed in 1% agarose and visualized with ethidium bromide. The DNA bands associated with the bcr/abl transcript were then extracted and followed by DNA sequence analysis.

Results: Major bcr/abl transcripts of the b3a2 type and minor ones of the e1a2 type were found in one and two ET patients, respectively. The incidence of bcr/abl transcripts was 21.4% (three of 14 patients).

Conclusion: Our experiments confirmed that bcr/abl transcripts are present in some patients with essential thrombocythemia.

+ For reprints and all correspondence: Hironori Yamada, Department of Hematology, Nagoya National Hospital, 4–1–1 Sannomaru, Nakaku, Nagoya 460-0001, Japan. E-mail: hayabusa@kctv.ne.jp

§ Abbreviations: ET, essential thrombocythemia; CML, chronic myelogenous leukemia; MPD, myeloproliferative disorders; Ph1, Philadelphia; PVSG, Polycythemia Vera Study Group; NAP, neutrophil alkaline phosphatase; PN, patient number; PB, peripheral blood; BM, bone marrow; PBS, phosphate-buffered saline; cDNA, complementary DNA; XCIPs, X-chromosome inactivation patterns; MRD, minimal residual disease; AML, acute myelogenous leukemia.


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