Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (16)
Right arrow Request Permissions
Google Scholar
Right arrow Articles by Hiroyasu, S.
Right arrow Articles by Muto, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hiroyasu, S.
Right arrow Articles by Muto, Y.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Japanese Journal of Clinical Oncology 31:65-68 (2001)
© 2001 Foundation for Promotion of Cancer Research

Clinical Relevance of the Concentrations of Both Pyrimidine Nucleoside Phosphorylase (PyNPase) and Dihydropyrimidine Dehydrogenase (DPD) in Colorectal Cancer

Shungo Hiroyasu, Masayuki Shiraishi, Hironori Samura, Hideo Tokashiki, Hideaki Shimoji, Tsutomu Isa and Yoshihiro Muto+,§

First Department of Surgery, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan

Background: Pyrimidine nucleoside phosphorylase (PyNPase) converts 5'-deoxy-5-fluorouridine (5'-DFUR) to 5'-fluorouracil (5-FU), which exerts an anti-cancer effect before being catabolized by dihydropyrimidine dehydrogenase (DPD). We examined the possible correlation of the tissue concentrations of both PyNPase and DPD with the clinicopathological features of colorectal cancer.

Methods: In 36 cases of colorectal cancer, the concentrations of both PyNPase and DPD in fresh-frozen samples from either tumor or normal tissue were quantified using ELISA.

Results: The concentration of PyNPase was found to be significantly higher in the tumor than in the normal tissue (p = 0.001), whereas DPD showed no difference. The tumor/normal tissue ratio of PyNPase was higher in advanced stage cases, and also in the presence of liver metastasis, lymph node metastasis and vessel invasion (each p < 0.05). On the other hand, the tumor/normal tissue ratio of DPD was also higher in advanced stage cases and also in the presence of vessel invasion (each p < 0.05), thus indicating a poor response to 5-FU. The PyNPase/DPD ratio, which is known to be correlated with the tissue concentration of 5'-DFUR, was higher in the tumor than in the normal tissue (p = 0.001).

Conclusions: The tumor/normal tissue ratios of both PyNPase and DPD might be useful candidates for predicting the prognosis of colorectal cancer. The PyNPase/DPD ratio was higher in the tumor tissue than in the normal tissue; however, further investigations are needed to clarify the effectiveness of fluoropyrimidine therapy.

+ For reprints and all correspondence: Shungo Hiroyasu, First Department of Surgery, University of the Ryukyus, Uehara 207, Nishihara-cho, Okinawa 903-0125, Japan. E-mail: hiroyasu@med.u-ryukyu.ac.jp

§ Abbreviations: PyNPase, pyrimidine nucleoside phosphorylase; DPD, dihydropyrimidine dehydrogenase; 5'-DFUR, 5'-deoxy-5-fluorouridine/doxifluridine; 5-FU, 5'-fluorouracil; PD-ECGF, platelet-derived endothelial cell growth factor; MoAb, monoclonal antibody


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.