Japanese Journal of Clinical Oncology 32:162-166 (2002)
© 2002 Foundation for Promotion of Cancer Research
Nitric Oxide Levels and Lipid Peroxidation in Plasma of Patients with Gastric Cancer
1 Department of Biochemistry, School of Medicine, 2 Biotechnology Application and Research Center and 3 General Surgery, Atatürk University, Medical School, Erzurum, Turkey
Background: The aim was to investigate the levels of malondialdehyde and total NO2– plus NO3– marker for NO• generation in gastric carcinoma and to correlate their levels with the cancer stage.
Method: The pretreatment plasma samples were obtained from 38 patients with gastric cancer (seven patients at stage II, 19 at stage III and 12 at stage IV). Nitrite (NO2–) and nitrate (NO3–) levels, the end products of nitric oxide (NO•), were determined in these samples. NO2– was measured by using the Griess reaction and after enzymatic conversion of NO3– into NO2– by nitrate reductase, the resultant NO2– was also measured by the same method. Malondialdehyde (MDA), a lipid peroxidation marker, was measured by the thiobarbituric acid method.
Results: The levels of plasma MDA, NO• and NO3– were significantly higher in patients with gastric cancer compared with the healthy control group. Higher levels of MDA, NO• and NO3– were observed as the stage of the disease increased.
Conclusion: We found that increased NO• production and MDA levels were present in plasma of patients with gastric cancer. These increases can be associated with the oxidant–antioxidant status in these patients.
+ For reprints and all correspondence: Ebubekir Bakan, Ataturk University Medical School, Department of Biochemistry, 25240 Erzurum, Turkey. E-mail: ebubekirbakan@usa.net
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