Japanese Journal of Clinical Oncology 33:192-197 (2003)
© 2003 Foundation for Promotion of Cancer Research
Helicobacter Pylori Seropositivity and the Myeloperoxidase G-463A Polymorphism in Combination with Interleukin-1B C-31T in Japanese Health Checkup Examinees
1 Nagoya Kita Health Center, Nagoya, 2 Department of Preventive Medicine/Biostatistics and Medical Decision Making, Nagoya University Graduate School of Medicine, 3 Division of Epidemiology and Prevention, Aichi Cancer Center Research Institute, Aichi and 4 Aichi Cancer Center, Aichi, Japan
Objective: Genetic susceptibility appears to play an important role in Helicobacter pylori (HP) infection. The present study was conducted to re-examine the reported association between the myeloperoxidase (MPO) G-463A polymorphism and HP seropositivity in different subjects and to investigate interactions with smoking behavior and the interleukin-1B (IL-1B) C-31T polymorphism.
Methods: The subjects were 468 health checkup examinees in Nagoya, who consented to anonymous genotyping of residual blood samples. Genotyping was conducted by PCR-RFLP for MPO G-463A and PCR–CTPP for IL-1B C-31T.
Results: Among the successfully genotyped 437 participants without a cancer history, the HP seropositive rate was 56.2% for –463GG (n = 354), 49.4% for –463GA (n = 77) and 83.3% for –463AA (n = 6). The gender–age-adjusted odds ratio (aOR) for GA/AA relative to GG was 0.86 (95% confidence interval, 0.32–1.34) among males, 0.84 (0.47–1.49) among females, 0.83 (0.22–3.05) among current smokers and 0.87 (0.50–1.51) among never smokers. Analysis by IL-1B C-31T genotype revealed a significantly reduced OR of 0.41 (0.18–0.93) among the participants with IL-1B –31CT. The OR for IL-1B –31TT relative to –31CC/CT was 1.59 (1.00–2.55) among those with MPO –463GG and 3.36 (1.16–9.77) with MPO –463GA/AA. None of the gene–environment or gene–gene interactions proved to be statistically significant.
Conclusions: The association between MPO G-463A and HP seropositivity was not reproduced in this study. The effect of IL-1B –31TT was more prominent among individuals with the low expression MPO –463A allele, but it remains to be confirmed for other datasets.
+ For reprints and all correspondence: Nobuyuki Katsuda, Nagoya Kita Health Center, 4–17–1 Shimizu, Kita Ward, Nagoya 462-8522, Japan. E-mail: katsuda{at}nifty.com
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