Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (10)
Right arrow Request Permissions
Google Scholar
Right arrow Articles by Yang, Y.-Y.
Right arrow Articles by Yang, C.-C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yang, Y.-Y.
Right arrow Articles by Yang, C.-C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Japanese Journal of Clinical Oncology 34:176-183 (2004)
© 2004 Foundation for Promotion of Cancer Research

Involvement of Viral and Chemical Factors with Oral Cancer in Taiwan

Yu-Yen Yang1, Lim-Woh Koh2, Ju-Hsin Tsai3, Chung-Hung Tsai4, Eric Fook-Chuen Wong1, Shyh-Jye Lin5 and Chi-Chiang Yang5,+

1 Department of Medical Research and 2 Department of Obstetrics and Gynecology, Show Chwan Memorial Hospital, Changhua, Taiwan, 3 Department of Surgery and 4 Department of Pathology, Chung Shan Medical University Hospital and 5 School of Medical Technology, Chung Shan Medical University, Taichung, Taiwan, ROC

Background: The association between oral squamous cell carcinoma (OSCC) and viral and chemical factors is uncertain. Therefore the correlation of viral and chemical factors with oral cancer in Taiwan was investigated.

Methods: Thirty-seven paraffin-embedded oral cancer biopsies and 36 normal oral tissue specimens were examined by the polymerase chain reaction method for six viruses: HPV, CMV, EBV, HSV-1, HSV-2 and HHV-8. To elucidate the role of arecoline in the oncogenesis of oral cancer, human buccal fibroblasts, oral submucosal fibroblasts and three cancer cell lines KB, GNM and TSCCa were used for MTT cytotoxity assay and flow cytometry DNA content analysis.

Results: Two (5.4%) HSV-1-positive and four (10.8%) HPV-positive cases were recognized in oral cancer biopsies. Among the four HPV-positive tissues, two were further typed as HPV-16, one was identified as HPV-18- and HSV-1-positive; and one contained both HPV-16 and HPV-18. One sample presented HSV-1 only. Arecoline, at a concentration lower than 0.8 µg/ml, increased cell growth (all cell types); at higher concentrations (25–400 µg/ml) it was cytotoxic. The cell cycle was demonstrated to be altered either by low or high concentrations of arecoline treatment, depending on the cells treated.

Conclusions: The data demonstrated that HPV, HSV-1 and betel quid chewing were significantly associated with OSCC, but HSV-2, CMV, EBV and HHV-8 were not. We suggest that the most determinative factor for oral cancer may be chemical in nature rather than viral infection.

+ For reprints and all correspondence: Chi-Chiang Yang, School of Medical Technology, Chung Shan Medical University, 110, Section 1, Chien-Kuo North Road, Taichung, Taiwan 402, ROC. E-mail: cyang{at}csmu.edu.tw


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Ann OncolHome page
N. Termine, V. Panzarella, S. Falaschini, A. Russo, D. Matranga, L. Lo Muzio, and G. Campisi
HPV in oral squamous cell carcinoma vs head and neck squamous cell carcinoma biopsies: a meta-analysis (1988-2007)
Ann. Onc., October 1, 2008; 19(10): 1681 - 1690.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.