Japanese Journal of Clinical Oncology Advance Access originally published online on November 28, 2005
Japanese Journal of Clinical Oncology 2005 35(12):739-744; doi:10.1093/jjco/hyi195
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© 2005 Foundation for Promotion of Cancer Research
Clinicopathologic and Prognostic Significance of MMP-7 (Matrilysin) Expression in Human Rectal Cancer
1 Department of Gastrointestinal Surgery, 2 Institute of Digestive Surgery and Organ Microcirculation and 3 National Key Laboratory of Biotherapy of West China Hospital, Sichuan University, Chengdu, Sichuan Province, China
For reprints and all correspondence: Zong-guang Zhou, Department of Gastrointestinal Surgery, West China Hospital, Sichuan University, 37 Guo Xue Xiang, Chengdu 610041, Sichuan Province, China. E-mail: Zhou767{at}21cn.com
Received June 28, 2005; accepted October 9, 2005
Objective: There is increasing evidence to indicate that MMP-7 plays a more important role in tumor progression than other MMPs. The aim of this study was to detect MMP-7 expression in human rectal cancer and normal rectal tissue and to determine whether it is correlated with invasion and metastasis of human rectal cancer.
Methods: Eighty-six paired samples of rectal cancer and distant normal rectal tissue obtained from 100 inpatients were allocated into two groups (cancer group and control group). MMP-7 mRNA was detected by relative quantitative real-time RT–PCR and MMP-7 protein was examined by immunohistochemical staining and computerized image analysis.
Results: MMP-7 mRNA expression in cancer group was higher than that in control group (P = 0.006), the expression ratios of 31 samples (37.35%) were <1 and 52 (62.65%) were >1. The mRNA expression level was correlated with Dukes Staging, histological differentiation grade and CEA level. The MMP-7 protein expression was in accordance with mRNA expression level. The positive degree of immunohistochemical staining in cancer group (1.82 ± 0.03) was different from that in control group (1.17 ± 0.13, P = 0.002). Moreover, in cancer group the positive staining degree in high-level mRNA cancers (2.04 ± 0.18, n = 52) was higher than that in low-level mRNA ones (1.58 ± 0.23, n = 31, P = 0.008).
Conclusions: Our results suggest that MMP-7 plays an important role in the progression of human rectal cancer. MMP-7 may be selected as a clinical diagnosis and prognosis index in rectal cancer.
Key Words: MMP-7 • expression • rectal cancer
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