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Japanese Journal of Clinical Oncology Advance Access originally published online on March 6, 2006
Japanese Journal of Clinical Oncology 2006 36(3):142-149; doi:10.1093/jjco/hyi246
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© 2006 Foundation for Promotion of Cancer Research

Cyclin E Expression in Operable Breast Cancer Quantified Using Real-Time RT–PCR: A Comparative Study with Immunostaining

Piotr Potemski1, Elzbieta Pluciennik2,3, Andrzej K. Bednarek3, Renata Kusinska2, Dorota Jesionek-Kupnicka2, Grazyna Pasz-Walczak2, Cezary Watala4 and Radzislaw Kordek2

1 Departments of Chemotherapy, 2 Pathology, 3 Molecular Cancerogenesis and 4 Haemostatic Disorders, Medical University of Lodz, Lodz, Poland

For reprints and all correspondence: Piotr Potemski, Department of Chemotherapy, Medical University of Lodz, 4 Paderewski Street, 93-509 Lodz, Poland. E-mail: piotrpo{at}mp.pl

Received November 7, 2005; accepted December 20, 2005

Objective: The main purpose of this retrospective study was to compare cyclin E expression levels in operable breast cancer patients determined using real-time RT–PCR and immunostaining. The prognostic relevance of cyclin E was also investigated.

Methods: Specimens of invasive ductal breast cancer tissues obtained from 124 women during radical mastectomy were analyzed.

Results: Of the tumor samples, 40.3 and 59.7% showed high expression of cyclin E in RT–PCR and immunostaining, respectively. The overall agreement probability was 0.032 according to Scott's {pi} statistic. With a median follow-up of 55.5 months, cyclin E expression assessed using immunostaining was an independent negative prognostic factor in the node positive group (hazard ratio 3.1; 95% CI 1.0–9.2; P = 0.045). Cyclin E expression correlated with absence of steroid receptors and younger age. RT–PCR results did not predict survival in any group of patients.

Conclusions: Disagreement between real-time RT–PCR and immunostaining was demonstrated. Immunostaining seems to be the more reliable method for assessing cyclin E in breast cancer cells.

Key Words: breast neoplasms • cyclin E • immunochemistry • prognosis • reverse transcription PCR


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