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Japanese Journal of Clinical Oncology Advance Access originally published online on August 12, 2009
Japanese Journal of Clinical Oncology 2009 39(11):771-775; doi:10.1093/jjco/hyp088
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© The Author (2009). Published by Oxford University Press. All rights reserved

Flow Cytometric Detection of Small Cell Lung Cancer Cells with Aberrant CD45 Expression in Micrometastatic Bone Marrow

Mikio Danbara1, Mitsugu Yoshida2, Yuhsaku Kanoh3, Shi-Xu Jiang4, Noriyuki Masuda5, Tohru Akahoshi6 and Masaaki Higashihara1

1 Department of Hematology, Kitasato University School of Medicine
2 Department of Clinical Laboratory, Kitasato University Hospital
3 Department of Laboratory Medicine, Kitasato University School of Medicine
4 Department of Pathology, Kitasato University School of Medicine
5 Department of Respiratory Medicine, Kitasato University School of Medicine
6 Department of General Medicine, Kitasato University School of Medicine, Kanagawa, Japan

For reprints and all correspondence: Mikio Danbara, Department of Hematology, Kitasato University School of Medicine, 1-15-1, Kitasato, Sagamihara, Kanagawa 228-8555, Japan. E-mail: mikduty{at}med.kitasato-u.ac.jp

Received April 7, 2009; accepted July 7, 2009

A lot of hematologists are often faced with the difficulty of diagnosing bone marrow micrometastasis of carcinoma cells. We employed a new flow cytometric immunophenotyping by a combination of CD45 with three neuroendocrine markers: CD56, microtubule-associated protein-2 and synaptophysin, and successfully detected micrometastatic tumor cells in the bone marrow of a 61-year-old male patient with small cell lung cancer (SCLC), whose marrow smears never showed a distinct morphology of metastasis. It was noteworthy that these SCLC cells accompanied the aberrant expression of CD45, leukocyte common antigen known as a specific marker for hematolymphoid neoplasms, which was not detected in the tumor of primary lesion. We describe this rare case to arouse an attention that tumors of non-hematolymphoid origin can exhibit exceptional CD45-positvity in metastatic sites.

Key Words: bone marrow micrometastasis • CD45 • flow cytometry • neuroendocrine • small cell lung cancer


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