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Japanese Journal of Clinical Oncology 21:330-333 (1991)
© 1991 Foundation for Promotion of Cancer Research

research-article

In Vitro Induction of Perforin mRNA and Cytotoxic Activities in Human Splenocytes by the Streptococcal Preparation, OK-432

Kazuhiko Kataoka*, Yoshio Naomoto and Kunzo Orita

First Department of Surgery, Okayama University Medical School 5-1, Shikata-cho 2-chome, Okayama 700

*For reprints and all correspondence (Present adress: Department of Surgery, National Matsudo Hospital 123-1, Takatsuka-Shinden, Matsudo 271)

Received February 20, 1991; accepted May 31, 1991

Mononuclear cells were isolated from a spleen excised during surgery and cultured with 0.05 KE (Klinische Einheit)/ml of the streptococcal preparation, OK-432, or 700 JRU (Japan Reference Units)/ml of recombinant interleukin-2 (rIL-2). When the cytotoxicity against K562 and Daudi cells was measured by four-hour 51Cr release assay, OK-432 induced about the same killing activity as that of rIL-2. The peak activity was observed on day 2. When the expression of human perforin messenger RNA (mRNA) was investigated by Northern blot analysis, a low-grade expression was seen in the splenocytes shortly after isolation (day 0). An expression 2.7-fold stronger than that seen on day 0 was produced after one day of culturing with OK-432. This enhanced expression decreased only slightly after the first day. The increases in expression of perforin mRNA on day 2 were similar for OK-432 and rIL-2. The results indicate the multicytokine inducer, OK-432, to induce perforin to about the same degree as rIL-2 does, and perforin to act as one of the effector molecules in the killing activity induced by OK-432.

Key Words: OK-432 • Spleen mononulcear • cells • Perforin • Northern blotting


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