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Japanese Journal of Clinical Oncology 30:301-305 (2000)
© 2000 Foundation for Promotion of Cancer Research

Characterization of Intracellular Cytokine Profile of CD4(+) T Cells in Peripheral Blood and Tumor-draining Lymph Nodes of Patients with Gastrointestinal Cancer

Hiroshi Nakayama1, Joji Kitayama1, Tetsuichiro Muto2 and Hirokazu Nagawa1,+

1Department of Surgery, Division of Surgical Oncology, The University of Tokyo and 2Cancer Institute Hospital, Tokyo, Japan

Background: Analysis of serum cytokine levels has shown that cancer-bearing hosts have lower levels of IL-2 and IFN-{gamma}, suggesting that Th1-type immunity is impaired by cancer. However, the mechanisms of the Th1 dysfunction are not clearly understood.

Method: The frequencies of Th1 cells in CD4(+) helper T cells were evaluated with an intra­cytoplasmic cytokine staining method in peripheral blood lymphocytes (PBL) and lymph node lymphocytes (LNL) of patients with gastrointestinal cancer.

Results: Activation of lymphocytes with PMA + Ionomycin induced the expression of IL-2 and IFN-{gamma} in each lymphocyte population. Compared with PBL of non-malignant donors, PBL in cancer patients contained significantly lower frequencies of CD4(+) T cells that produced IL-2 and IFN-{gamma}. LNL in cancer patients also contained lower levels of IL-2- and IFN-{gamma}-producing CD4(+) T cells, although the percentages did not show significant differences from those of PBL in the same patients.

Conclusion: Our data suggest that suppression of Th1 cytokine in cancer patients is,at least in part, due to the decreased frequency of Th1 cells with CD4(+) phenotype.

+ For reprints and all correspondence: Joji Kitayama, Department of Surgery, Division of Surgical Oncology, The University of Tokyo, Hongo 7–3–1, Bunkyo-ku, Tokyo 113-8655, Japan. E-mail: kitayama-1su@h.u-tokyo.ac.jp


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