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Japanese Journal of Clinical Oncology 32:219-221 (2002)
© 2002 Foundation for Promotion of Cancer Research

Concurrent Mutations of K-ras Oncogene at Codons 12 and 22 in Colon Cancer

Yasuyuki Miyakura1,2, Kokichi Sugano2, Noriko Fukayama2, Fumio Konishi3 and Hideo Nagai1,+

1 Department of Surgery, Jichi Medical School, Minami Kawachi, Tochigi, 2 Oncogene Research Unit/Cancer Prevention Unit, Tochigi Cancer Center Research Institute, Utsunomiya and 3 Department of Surgery, Jichi Medical School Omiya Medical Center, Saitama, Japan

K-ras mutation is the most common oncogenic alteration in various human cancers including colorectal carcinomas. Point mutations have the potential to activate the K-ras gene if they occur in the critical coding sequences. Almost all of these mutations have been localized in codons 12, 13 and 61. We report a case of colon cancer presenting point mutations at both codons 12 and 22 of the K-ras gene. PCR-SSCP and subsequent sequencing revealed that GGT (glycine, wild-type) to AGT (serine) substitution at codon 12 and CAG (glutamine, wild-type) to CGG (arginine) substitution at codon 22 occurred in the same allele.

+ For reprints and all correspondence: K. Sugano, Oncogene Research Unit/Cancer Prevention Unit, Tochigi Cancer Center Research Institute, 4–9–13 Yohnan, Utsunomiya, Tochigi 320-0834, Japan


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