Japanese Journal of Clinical Oncology 32:238-243 (2002)
© 2002 Foundation for Promotion of Cancer Research
Decreased Peroxisome Proliferator-activated Receptor Gamma Gene Expression is Correlated with Poor Prognosis in Patients with Esophageal Cancer
Department of Surgery II, Nagoya City University Medical School, Nagoya, Japan
Background: Peroxisome proliferator-activated receptor gamma (PPAR
) induces apoptosis by ligand stimulation in various tumor cell lines. In esophageal cancer cell lines, PPAR activation also has suppressed the proliferation.
Methods: In 55 primary esophageal squamous cell carcinomas (ESCCs) we examined the correlation between the expression of PPAR mRNA with prognosis of esophageal cancer patients. The expression of PPAR mRNA was quantified by real-time reverse transcription polymerase chain reaction using LightCycler. Immunohistochemistry was used to study the expression of PPAR protein.
Results: The expression of PPAR mRNA was significantly decreased in esophageal cancer cells compared with normal esophageal mucosa (P = 0.0084). Among the clinical factors, PPAR mRNA expression was lower in the tumors with extensive lymph node metastasis (n4) than those with less extensive lymph node metastasis (n0–3) (P = 0.0059). Patients with low PPAR mRNA expression had significantly shorter postoperative survival time than those with high PPAR mRNA expression (P = 0.0191). In immunohistochemistry, PPAR protein was expressed in the nuclei of cells in some cases and expressed in the nuclei and cytoplasm in others. The expression of PPAR protein is decreased in esophageal cancer tissue compared with normal esophageal squamous epithelium. However, we could not deduce the apparent relation for the expression between PPAR mRNA and PPAR proteins in immunohistochemistry (P = 0.284).
Conclusions: In esophageal cancer tissues, the expression of PPAR was decreased compared with normal esophageal epithelium. The mRNA expression level of PPAR may be a marker of prognosis after operation in esophageal cancer patients.
+ For reprints and all correspondence: Yoshitaka Fujii, Department of Surgery II, Nagoya City University Medical School, 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 467-8601, Japan. E-mail: yosfujii@med.nagoya-cu.ac.jp
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