Japanese Journal of Clinical Oncology Advance Access published online on November 28, 2005
Japanese Journal of Clinical Oncology, doi:10.1093/jjco/hyi195
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1 Department of Gastrointestinal Surgery, Sichuan University, Chengdu, Sichuan Province, China
* To whom correspondence should be addressed. Objective: There is increasing evidence to indicate that MMP-7 plays a more important role in tumor progression than other MMPs. The aim of this study was to detect MMP-7 expression in human rectal cancer and normal rectal tissue and to determine whether it is correlated with invasion and metastasis of human rectal cancer. Methods: Eighty-six paired samples of rectal cancer and distant normal rectal tissue obtained from 100 inpatients were allocated into two groups (cancer group and control group). MMP-7 mRNA was detected by relative quantitative real-time RT-PCR and MMP-7 protein was examined by immunohistochemical staining and computerized image analysis. Results: MMP-7 mRNA expression in cancer group was higher than that in control group (P = 0.006), the expression ratios of 31 samples (37.35%) were <1 and 52 (62.65%) were >1. The mRNA expression level was correlated with Dukes Staging, histological differentiation grade and CEA level. The MMP-7 protein expression was in accordance with mRNA expression level. The positive degree of immunohistochemical staining in cancer group (1.82 ± 0.03) was different from that in control group (1.17 ± 0.13, P = 0.002). Moreover, in cancer group the positive staining degree in high-level mRNA cancers (2.04 ± 0.18, n = 52) was higher than that in low-level mRNA ones (1.58 ± 0.23, n = 31, P = 0.008). Conclusions: Our results suggest that MMP-7 plays an important role in the progression of human rectal cancer. MMP-7 may be selected as a clinical diagnosis and prognosis index in rectal cancer.
Received June 28, 2005
Accepted October 9, 2005
Original Article
Clinicopathologic and Prognostic Significance of MMP-7 (Matrilysin) Expression in Human Rectal Cancer
Hong-zhi Luo 1,
Zong-guang Zhou 2 *,
Lie Yang 1,
Yong-yang Yu 1,
Cao Tian 1,
Bing Zhou 3,
Xue-Lian Zheng 3,
Qing-jie Xia 3,
Yuan Li 3,
and
Rong Wang 3
2 Department of Gastrointestinal Surgery, Sichuan University, Chengdu, Sichuan Province, China; Institute of Digestive Surgery and Organ Microcirculation, Sichuan University, Chengdu, Sichuan Province, China; National Key Laboratory of Biotherapy of West China Hospital, Sichuan University, Chengdu, Sichuan Province, China
3 Institute of Digestive Surgery and Organ Microcirculation, Sichuan University, Chengdu, Sichuan Province, China
Zong-guang Zhou, E-mail: Zhou767{at}21cn.com
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