Japanese Journal of Clinical Oncology Advance Access published online on February 1, 2006
Japanese Journal of Clinical Oncology, doi:10.1093/jjco/hyi227
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Department of Pathology, Hamamatsu University School of Medicine, Hamamatsu, Shizuoka, Japan; Department of Surgery, Hamamatsu University School of Medicine, Hamamatsu, Shizuoka, Japan
* To whom correspondence should be addressed. Background: Biological variations in and the heterogeneity of gastrointestinal stromal tumors (GISTs) are well known, but chromosomal numerical abnormality (CNA) has not been fully examined especially in this context. The aim of this study is to test CNA as a possible biological predictor of biological behavior of GISTs. Method: We applied microwave-assisted FISH protocol to pathological archives of GIST tumors displaying different clinical features to characterize the CNA profile of these tumors. A panel of 18 centromere enumeration probes (CEP) and 24 bacterial artificial chromosome (BAC) or P1-derived artificial chromosome (PAC) probes containing genes like Aurora kinases (AURKs) and other candidate genes involved in human carcinogenesis were used. CNA profiles, histopathological risk categorization and Ki-67 labeling indexes of 23 primary and/or metastatic GIST tumors of 12 subjects (both primary and metastatic in 7 subjects) were compared between primary GIST with and without metastases, and between metastatic and primary portions in 7 individuals. Results: CNA in the primary sites was more extensive in the GISTs with recurrence and metastasis than in those without, especially as to the loss of chromosome 20 and genomic imbalance of AURKA-containing BAC probe on 20q in the cases with metastasis. The consistent loss of one allele of chromosome 14q was also noted. Interestingly, both primary and metastatic tumors in identical individuals had similar CNA profiles. Conclusion: The extent of CNA differed between GISTS with and without recurrence or metastasis; thus, FISH analysis of specimens from the primary sites may predict the biological behavior of this tumor.
Received June 17, 2005
Accepted December 1, 2005
Original Article
Chromosomal Numerical Abnormality Profiles of Gastrointestinal Stromal Tumors
Kimihiro Yamashita 1,
Hisaki Igarashi 2,
Yasuhiko Kitayama 3,
Takachika Ozawa 4,
Shinichiro Kiyose 5,
Hiroyuki Konno 6,
Teruhisa Kazui 7,
Shumpei Ishikawa 8,
Hiroyuki Aburatani 8,
Fumihiko Tanioka 9,
Masaya Suzuki 10,
and
Haruhiko Sugimura 2 *
2 Department of Pathology, Hamamatsu University School of Medicine, Hamamatsu, Shizuoka, Japan
3 Department of Pathology, Hamamatsu University School of Medicine, Hamamatsu, Shizuoka, Japan; Department of Pathology, Shizuoka Saiseikai General Hospital, Shizuoka, Japan
4 Department of Pathology, Hamamatsu Medical Center, Hamamatsu, Shizuoka, Japan
5 JOKO Corporation, Hongo, Bunkyo-ku, Tokyo, Japan
6 Department of Digestive Surgery, Hamamatsu University School of Medicine, Hamamatsu, Shizuoka, Japan
7 Department of Surgery, Hamamatsu University School of Medicine, Hamamatsu, Shizuoka, Japan
8 Department of Genome Science, RCAST, Tokyo, Japan
9 Department of Pathology, Hamamatsu University School of Medicine, Hamamatsu, Shizuoka, Japan; Department of Pathology and Laboratory Medicine, Iwata City Hospital, Iwata, Shizuoka, Japan
10 Department of Pathology, Hamamatsu University School of Medicine, Hamamatsu, Shizuoka, Japan; Information System Division, Biosafety Research Center, Foods, Drugs, and Pesticides, Iwata, Shizuoka, Japan
Haruhiko Sugimura, E-mail: hsugimur{at}hama-med.ac.jp
Abstract 
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  H. Sugimura Detection of chromosome changes in pathology archives: an application of microwave-assisted fluorescence in situ hybridization to human carcinogenesis studies Carcinogenesis, April 1, 2008; 29(4): 681 - 687. [Abstract] [Full Text] [PDF]
|
|