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Japanese Journal of Clinical Oncology Advance Access published online on March 6, 2006
Japanese Journal of Clinical Oncology, doi:10.1093/jjco/hyi246
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© 2006 Foundation for Promotion of Cancer Research
Received November 7, 2005
Accepted December 20, 2005

Original Article

Cyclin E Expression in Operable Breast Cancer Quantified Using Real-Time RT-PCR: A Comparative Study with Immunostaining

Piotr Potemski 1 *, Elzbieta Pluciennik 2, Andrzej K. Bednarek 3, Renata Kusinska 4, Dorota Jesionek-Kupnicka 4, Grazyna Pasz-Walczak 4, Cezary Watala 5, and Radzislaw Kordek 4

1 Department of Chemotherapy, Medical University of Lodz, Lodz, Poland
2 Department of Pathology, Medical University of Lodz, Lodz, Poland; Department of Molecular Cancerogenesis, Medical University of Lodz, Lodz, Poland
3 Department of Molecular Cancerogenesis, Medical University of Lodz, Lodz, Poland
4 Department of Pathology, Medical University of Lodz, Lodz, Poland
5 Department of Haemostatic Disorders, Medical University of Lodz, Lodz, Poland

* To whom correspondence should be addressed.
Piotr Potemski, E-mail: piotrpo{at}mp.pl


  Abstract

Objective: The main purpose of this retrospective study was to compare cyclin E expression levels in operable breast cancer patients determined using real-time RT-PCR and immunostaining. The prognostic relevance of cyclin E was also investigated.

Methods: Specimens of invasive ductal breast cancer tissues obtained from 124 women during radical mastectomy were analyzed.

Results: Of the tumor samples, 40.3 and 59.7% showed high expression of cyclin E in RT-PCR and immunostaining, respectively. The overall agreement probability was 0.032 according to Scott's {pi} statistic. With a median follow-up of 55.5 months, cyclin E expression assessed using immunostaining was an independent negative prognostic factor in the node positive group (hazard ratio 3.1; 95% CI 1.0-9.2; P = 0.045). Cyclin E expression correlated with absence of steroid receptors and younger age. RT-PCR results did not predict survival in any group of patients.

Conclusions: Disagreement between real-time RT-PCR and immunostaining was demonstrated. Immunostaining seems to be the more reliable method for assessing cyclin E in breast cancer cells.

Keywords: breast neoplasms; cyclin E; immunochemistry; prognosis; reverse transcription PCR.
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