Skip Navigation



Japanese Journal of Clinical Oncology Advance Access published online on January 19, 2007
Japanese Journal of Clinical Oncology, doi:10.1093/jjco/hyl137
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
37/2/127    most recent
hyl137v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by Yang, Q.-C.
Right arrow Articles by Huang, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yang, Q.-C.
Right arrow Articles by Huang, J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2007 Foundation for Promotion of Cancer Research

Overexpression of Hypoxia-Inducible Factor-1{alpha} in Human Osteosarcoma: Correlation with Clinicopathological Parameters and Survival Outcome

Qing-Cheng Yang1, Bing-Fang Zeng1,, Yang Dong1, Zhong-Min Shi1, Zhi-Ming Jiang2 and Jin Huang2

1 Department of Orthopedics
2 Department of Pathology, Shanghai Sixth People's Hospital of Shanghai JiaoTong University, Shanghai, China

For reprints and all correspondence: Bing-Fang Zeng, Department of Orthopedics, Shanghai Sixth People's Hospital of Shanghai JiaoTong University, Yishan Road No. 600, Shanghai, 200233, China. E-mail: bingfangzeng{at}yahoo.com.cn

Received May 4, 2006; accepted September 29, 2006

BACKGROUND: Hypoxia is a common feature of many solid cancers and linked to malignant transformation, metastases and treatment resistance. Hypoxia is known to induce hypoxia-inducible factor-1{alpha} (HIF-1{alpha}) expression. The aim of this study is to investigate the impact of overexpression of HIF-1{alpha} on prognosis and the relationship with clinicopathological characteristics in human osteosarcoma.

METHODS: Immunochemistry with digital image analysis was used to determine the HIF-1{alpha} protein expression in histologic sections from 39 treated patients.

RESULTS: According to our study, expression of HIF-1{alpha} protein were detected in 31 of 39 cases (79%), with signal concentrated primarily within the nuclei of tumor cell. In contrast, non-cancerous adjacent tissues showed no HIF-1{alpha} immunoreactivity. HIF-1{alpha} expression was significantly associated with surgical stage, percentage of dead cells and microvessel density (MVD). Surgical stage, percentage of dead cells and HIF-1{alpha} expression showed significant influence on overall survival (OS) and disease-free survival (DFS) in univariate analysis. In multivariate analysis, surgical stage (IIA versus IIB/III) and percentage of dead cells (<90% versus ≥ 90%) were significant for DFS and OS. Those patients with HIF-1{alpha} moderate/strong expression showed significantly shorter OS and DFS compared with HIF-1{alpha} negative/weak expression.

CONCLUSIONS: Overexpression of HIF-1{alpha} is predictive of a poor outcome and might be a novel therapeutic target in human osteosarcoma.

Key Words: osteosarcoma • HIF-1{alpha} • prognosis • immunochemistry


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.